Investigations on Fungistatic Activity of Human and Animal Sera on Dermatophyte Grow
DOI:
https://doi.org/10.5644/Radovi.82Abstract
Investigations have been carried out regarding the fungistatic activity of human and animal sera on certain species of dermatophytes (T. tonsurans, T. schoenleini, T. violaceum, and T. mentagrophytes).
The tests were performed with active and inactivated sera (lh at 56° C) in different concentrations: 100%, 80%, 60%, 40%, and 20%. As diluent and control has served 1% pepton.
As inocula were used standard volumina of fungi elements in parasitic State derived from pathological material (infected hairs) and (using separate sera but from the same individuals) inocula of fungi in saprophytic State derived from 15 days old cultures. Sera were incubated at room temperature (approx. 25° C). Results of the tests were registered on the fifth and tenth day after inoculation.
There were investigated 28 human sera from persons infected with tinea favosa and with different trichophyton infections (superficial, generalised, kerion). Sera from healthy persons were also included. Animal sera were from healthy horse and cattle.
The results obtained from these investigations show that:
a) human and animal sera are giving definite evidence of fungistatic activity on tested dermatophytes;
b) the inhibitory effect is more pronounced using inocula from pathologic material (fungi in parasitic state) than inocula which originated from fungi in saprophytic state (culture);
c) there are no well-marked macroscopical differences in inhibition of dermatophyte growth between active and inactivated sera. However, by culturing the inhibited inocula on Saboraud 2% glukoze medium defined differences could be noticed between the inocula inhibited by active sera and inactivated. Inocula inhibited by inactivated sera show a more intensive and luxuriant growth and the microscopical morphology is more rich than in the inocula inhibited by active sera. This difference was especially marked by T. schoenleini;
d) it could be evidenced an unequal fungistatic activity of the same sera on different species of investigated dermatophytes. Human sera showed the most intensive fungistatic activity on T. tonsurans (parasitic state), then follow in order of decreased sensitivity T. schoenleini and T. violaceum. The lowest fungistatic effect of human sera was exerted on T. mentagrophytes (granular type). In general, human sera demonstrated a more intensive fungistatic activity than sera from tested animals;
e) the fungistatic effect of human and animal sera cannot be regarded as caused by specific antibodies, but as a result of a common serum property of nonspecific nature;
f) there was not seen any difference in the inhibitory effect of sera whether they originated from healthy or infected persons.
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