Interpretation of the Find of Fluorescent Bacilli in Sputum Using the Auramine Microscopic Method
DOI:
https://doi.org/10.5644/Radovi.220Keywords:
Mycobacterium tuberculosis, Fluorescent microscopy, AuraminAbstract
We tested about 1.000 clinical specimens of M. tuberculosis by culture, Ziehl-Neelsen and new fluorescent microscopic method which use for staining the fluorochrome auramine. Our results, like ali other recent studies of other authors, show that the ne\v fluorescent microscopic method is more sensitive than classical Ziehl-Neelsen method.
Using the method of Buck and Gart we determined, when as criterion for a positive result we take the finding of minimum 10 rods, that the sensitivity of F1 method is 65%, its specificity 96% (in relation to reference culture method) and general agreement of positive and negative results by both methods is 92%.
When for positive result less than 10 rods are required, the number of positive specimens is greater. Concerning literature data about very high frequency of available form of M. tuberculosis found in specimens of well treated patients with pulmonary tuberculosis, and concerning the clinical origin of the specimens we studied, it can be supposed that some of our oligobacillary specimens which are positive by fluorescent microscopic method and negative by culture contain just available form of M. tuberculosis, which could speak in favour of still greater specificity and sensitivity of the new fluorescent microscopic method for diagnostics of M. tuberculosis.
References
Bennedsen, J. and Larsen, S. O. (1966): Examination for tubercle bacilli by fluorescence microscopy, Scand. J. Resp. Dis. 47:114—120.
Buck, A. A. and Gart, J. (1966): Comparaison of a screening test and a refer rence test in epidemiologic studies, Am. J. Epidemiol. 83:586—592.
Dobardžić, R. et al. (1976): Faktor vrijeme kod auraminske dijagnostičke metode tuberkuloze, Radovi XVIII, Naučni sastanak mikrobiologa i epidemiologa Jugoslavije (Pula), str. 745—748.
Fusillo, M. N. and Burns, H. D. (1968): Simultaneous auramine and Kinyoun stain for screaning smears for acid-fast bacilli, Am. J. Clin. Path., 49:753—754.
Glebović, O. B. et al. (1974): Luminescentnaia mikroskopiia BK v diagnostike tuberkuleza legkih, Voen. Med. Zh., 2:41—45.
Golubeev, D. B. et. al. (1969): Teoriia i praktika ekspres diagnostiki gripa i oštrih respiratornih virusnih zabolevanii metodom imunofluorescencii, izd. Ministarstvo zdravoohraneniia, Leningrad, str. 78—104.
Ippen, G. B. (1968): Sravnitelnaia ocenka nekotorih metodov viavleniia M. tuberkuleza pri specifičnom porazenii kostei i sustavov, Probi, tuberkul., 46:78—80.
Jascenko, T. N. i Mečeva, I. S. (1973): Rukovodstvo po laboratomim isledovaniam pri tuberkuleze, izd. Medicina, Moskva.
Parrot, R. et al. (1967): La microscopie en fluorescence du bacille tuberculeux, Rev. Tuberc. et Pneumol., 31:511—516.
Richter, J. and Halova, R. (1968): Quantitative comparaison between microscopical demonstration of tubercle bacilli by fluorescence method and the classical staining technique after Ziehl-Neelsen and with cultivation on liquid and solid media, Rozhl. Tuberk., 28:440—444.
Strahov, N. S. et al. (1973): Znacenie luminescentnoi mikroskopii v svoevremenoi diagnostike tuberkuleza, Probi, tuberkul., 4:69—71.
Tacquet, A. et Tison, F. (1973): Mycobactčries, u "Techniques en bacteriologie", 2:131—133. Flammarion, Paris.
Vestal, A. L. (1973): Procedures for the isolation and Identification of Mycobacteria, izd. USA Deprtment HEW-PHS, Atlanta.
Winblad, B. and Duchek, M. (1973): Comparaison between microscopical methods and cultivation for demonstration of BK in experimental tuberculous infection, Acta Path. Microbiol. Scand. A81:824—830.
*** Comitć OMS d'experts de la tuberculose, 9C rapport (1974).
Downloads
Published
Issue
Section
License
Copyright (c) 2026 R. Dobradžić, H. Serdarević, Azra Mahmutćehajić, Edita Sučić
This work is licensed under a Creative Commons Attribution 4.0 International License.
