Quantitative Analysis of CCL5 and ep300 in Periapical Inflammatory Lesions

Authors

  • Naida Hadziabdic Department of Oral Surgery, Faculty of Dental Medicine, University of Sarajevo, Sarajevo,
  • Amina Kurtovic-Kozaric Department of Pathology, Clinical Center of the University of Sarajevo, Sarajevo
  • Azra Frkatovic Department of Genetics and Bioengineering, International Burch University, Sarajevo
  • Samira Smajlovic Department of Genetics and Bioengineering, International Burch University, Sarajevo
  • Ariadne Letra Center for Craniofacial Research, Department of Diagnostic and Biomedical Sciences, School of Dentistry, University of Texas Health Science Center at Houston, Houston, TX 77054

DOI:

https://doi.org/10.5644/ama2006-124.251

Keywords:

Chemokine CCL5, E1AAssociated P300 Protein, Periapical Granuloma, Periapical Periodontitis, Radicular Cyst

Abstract

Objectives. In silico bioinformatical analysis suggested that the expression of two genes, CCL5 (C-C Motif Chemokine Receptor 5) and ep300 (Histone acetyltransferase p300), could be used as potential new biomarkers in differentiation between periapical granulomas and radicular cysts. Thus, we hypothesized that gene expression of CCL5 and ep300 in periapical lesions would classify the lesions as either granuloma or cyst.

Materials. Patient samples (n=122) included 46 periapical granulomas, 38 radicular cysts and 38 healthy gingival samples as controls. Real-time PCR analysis of CCL5 and ep300 transcripts was compared to SDHA (Succinate dehydrogenase complex, subunit A) as the reference. Clinical parameters (e.g., intensity of inflammation and lesion size) were measured and correlated with CCL5 and ep300 expression. ROC (Receiver operating characteristic) and logistic regression analyses were used to establish the diagnostic character of ΔCt values.

Results. Granulomas and radicular cysts had significantly higher expression of CCL5 and ep300 compared to controls (P<0.05). However, no differences were observed when comparing granulomas and radicular cysts. ROC analyses showed that CCL5 and ep300 have good diagnostic accuracy, but low accuracy for distinguishing between the lesions.

Conclusions. This study confirmed that expression of CCL5 and ep300 is relevant for the pathogenesis of periapical inflammatory lesions but cannot be used as a distinctive marker between these lesions.

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Published

2019-10-24

How to Cite

Hadziabdic, N., Kurtovic-Kozaric, A., Frkatovic, A., Smajlovic, S., & Letra, A. (2019). Quantitative Analysis of CCL5 and ep300 in Periapical Inflammatory Lesions. Acta Medica Academica, 48(2), 129–139. https://doi.org/10.5644/ama2006-124.251

Issue

Section

Clinical Science

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